Paul Voyizan, PhD

In ageing and disease, there is an increase in specific chemical modifications of proteins called glycoxidative post-translational modifications (GOX). Our research focuses on uncovering the mechanisms whereby these protein modifications cause pathology, specifically in bone. Studies include (i) mapping of GOX modifications in human bone matrix; (ii) determination of their effects on protein structure and function; (iii) characterization of global changes in bone matrix proteome related to GOX-modified proteins.

We also focus on development of novel translatable strategies to predict bone pathology based on identification of GOX-modified bone matrix peptides in serum and urine.

In our laboratory and through multiple collaborations we employ mass-spectrometry, high performance liquid chromatography, differential scanning calorimetry, thermogravimetric analysis and other technologies.

Research keywords: non-enzymatic glycoxidation; bone matrix; collagen; ageing; osteoporosis.

Selected publications (2020-2026):

Paul Voziyan, Kyle L. Brown, Sasidhar Uppuganti, Emily Berestesky, Micheal Leser, Kristie Lindsey Rose, Jeffry S. Nyman (2026) Sites of non-enzymatic crosslinks pentosidine and glucosepane in collagen I of human cortical bone. Bone. Apr 24:117908. doi: 10.1016/j.bone.2026.117908. Online ahead of print.

Berestesky, E., Uppuganti, S., Dapaah, D.Y., Fernandes, D., Livingston, N., Lutsky, D., Zhang, M., Hymel, A.M., Pennings, J., Voziyan, P., Does, M.D., Willett, T.L. & Nyman, J.S. (2026) Differences and Similarities in Cortical Bone of the Femur between Donors with and without Type 2 Diabetes. J Bone Miner Res. 41(4): 434-446. doi: 10.1093/jbmr/zjaf173.

Paul Voziyan, Kyle L. Brown, Sasidhar Uppuganti, Micheal Leser, Kristie Lindsey Rose, Jeffry S. Nyman (2024) A map of glycation and glycoxidation sites in collagen I of human cortical bone: Effects of sex and type 2 diabetes. Bone. 187: 117209. doi:10.1016/j.bone.2024.117209.

Uppuganti S, Creecy A, Fernandes D, Garrett K, Donovan K, Ahmed R, Voziyan P, Rendina-Ruedy E, Nyman JS. (2024) Bone Fragility in High Fat Diet-induced Obesity is Partially Independent of Type 2 Diabetes in Mice. Calcif Tissue Int. 115(3): 298-314. doi: 10.1007/s00223-024-01252-x.

Sergey V. Ivanov, Kristie L. Rose, Selene Colon, Billy G. Hudson, Gautam Bhave, Paul Voziyan (2023) Mechanism of peroxidasin inactivation in hyperglycemia: Heme damage by reactive oxygen species. Biochem. Biophys. Res. Commun. 689: 149237. doi: 10.1016/j.bbrc.2023.149237.

Sergey V. Ivanov, Kristie L. Rose, Selene Colon, Roberto M. Vanacore, Billy G. Hudson, Gautam Bhave, Paul Voziyan (2023) Identification of brominated proteins in renal extracellular matrix: Potential interactions with peroxidasin. Biochem. Biophys. Res. Commun. 681:152-156. doi: 10.1016/j.bbrc.2023.09.063.

M. Unal, S. Uppuganti, R. Ahmed, D.Y. Dapaah, J. Pennings, T. L. Willett, P. Voziyan, and J.S. Nyman (2023) Effect of Ribose Incubation on Physical, Chemical, and Mechanical Properties of Human Cortical Bone. J Mech Behav Biomed Mater. Apr; 140:105731. doi: 10.1016/j.jmbbm.2023.105731.

Paul Voziyan, Sasidhar Uppuganti, Micheal Leser, Kristie L. Rose, Jeffry S. Nyman (2023) Mapping glycation and glycoxidation sites in collagen I of human cortical bone. BBA Advances. 3: 100079. doi: 10.1016/j.bbadva.2023.100079.

Willett TL, Voziyan P, Nyman JS (2022) Causative or associative: A critical review of the role of advanced glycation end-products in bone fragility. Bone 163:116485. doi: 10.1016/j.bone.2022.116485.

Boudko SP, Bauer R, Chetyrkin SV, Ivanov S, Smith J, Voziyan PA, Hudson BG (2021) Collagen IVα345 dysfunction in glomerular basement membrane diseases. II. Crystal structure of the α345 hexamer. J Biol Chem. 296:100591. doi: 10.1016/j.jbc.2021.100591.

Pokidysheva EN, Seeger H, Pedchenko V, Chetyrkin S, Bergmann C, Abrahamson D, Cui ZW, Delpire E, Fervenza F, Fidler AL, Fogo AB, Gaspert A, Grohmann M, Gross O, Haddad G, Harris RC, Kashtan C, Kitching AR, Lorenzen JM, McAdoo S, Pusey CD, Segelmark M, Simmons A, Voziyan PA, Wagner T, Wüthrich RP, Zhao MH, Boudko SP, Kistler AD, Hudson BG (2021) Collagen IVα345 dysfunction in glomerular basement membrane diseases. I. Discovery of a COL4A3 variant in familial Goodpasture's and Alport diseases. J Biol Chem. 296: 100590. doi: 10.1016/j.jbc.2021.100590.

Creecy A, Brown KL, Rose KL, Voziyan P, Nyman JS (2021) Post-translational modifications in collagen type I of bone in a mouse model of aging. Bone 143: 115763. doi: 10.1016/j.bone.2020.115763.

He C, Song W, Weston TA, Tran C, Kurtz I, Zuckerman JE, Guagliardo P, Miner JH, Ivanov SV, Bougoure J, Hudson BG, Colon S, Voziyan PA, Bhave G, Fong LG, Young SG, Jiang H. (2020) Peroxidasin-mediated bromine enrichment of basement membranes. Proc Natl Acad Sci U S A, 117(27):15827-15836. doi: 10.1073/pnas.2007749117.