The Analytical Services Metabolic Biomarkers Core facilitates diabetes, endocrine and metabolic research across a range of species. Sensitive, reproducible, and inexpensive analysis of hormones, lipids, amino acids, stable isotopes and markers of oxidative stress and inflammation is provided. Investigators are given access to assays which are not readily available, assistance with method development, training, technical support and access to research space and personnel.
Managing Director: Dale Edgerton, PhD (dale.edgerton@vanderbilt.edu)
Director: Sean Davies, PhD (sean.davies@vanderbilt.edu)
Facility Manager: Susan Hajizadeh (susan.hajizadeh@vumc.org)
Administrative Officer: Alisa Escue (alisa.escue@vumc.org)
RIA / HPLC / Luminex / Enzymatic / ELISA / GC-MS assays:
- Radioimmunoassays (RIA): corticosterone, cortisol, c-peptide, ghrelin, glucagon, insulin, leptin, PYY and testosterone
- High Performance Liquid Chromatography (HPLC): amino acids and catecholamines (epinephrine & norepinephrine)
- Luminex multiplex: includes human, mouse and canine adipokine, cytokine/chemokine, metabolic hormones, IGF, immunology/inflammation, and pituitary panels
- Enzymatic: glucose
- ELISA: glucagon
- GC-MS: Atrial natriuretic peptide (ANP) & B-type natriuretic peptide (BNP)
* For inquiries, please contact the hormone core staff at (615) 936-1626 or email Dale Edgerton
LIPID ASSAYS:
- Enzymatic Lipid Profiles (plasma):
- cholesterol, triglycerides, HDL cholesterol, free fatty acids
- Gas Chromatography (GC) Lipid Analysis (tissue, cell culture, plasma):
- phospholipids, diglycerides, free fatty acids, triglycerides, cholesterol esters (includes the fatty acid profile and quantitation of each lipid class)
- total cholesterol, unesterified cholesterol (quantitation only)
- Fast Protein Liquid Chromatography (FPLC) Lipoprotein Separation (plasma):
- lipoprotein profile (includes plasma total cholesterol and triglycerides)
* For inquiries, contact the hormone core staff at (615) 343-2645 or email Carla Harris
***This core is supported by NIH grant DK020593 (DRTC). Please acknowledge this in your publications.***
