General Methods

Hormones - RIA

  • Used to measure ACTH, corticosterone, cortisol, c-peptide, ghrelin, glucagon, growth hormone, insulin, leptin, PYY, testosterone, T3 and T4
  • Hormones are assayed by RIA using a double antibody procedure, allowing for the detection of minute concentrations of biological (hormones) or pharmacological substances in blood or other fluid samples using antigen/antibody reactions.
  • The method provides a very sensitive, inexpensive method of measurement.
  • Final analysis is accomplished by quantifying the bound radioactive counts with a Packard Gamma counter connected to a computerized data reduction station.
  • These assays are high volume and their major benefit is in accuracy and cost savings.
  • See the Assays page for vendor information 
  • See the Sample Prep page for information about sample collection / required additives

Catecholamines - HPLC

  • Plasma norepinephrine and epinephrine are routinely measured by HPLC via electrochemical detection. Dopamine is not measured. 
  • Plasma is absorbed onto alumina at a ph of 8.6, eluted with dilute perchloric acid, and auto-injected onto a c18 reversed-phase column.
  • An internal standard (dehydroxylbenzylamine/DHBA) is included with each extraction to monitor recovery and aid in quantification.
  • Results are quantified through a chromatography data station.
  • This service is widely used and provides a precise and sensitive estimate of catecholamine levels in biologic tissues and fluids.
  • Catecholamines are unstable. See the Sample Prep page for information about sample collection, required additives, and tissue prep.

Amino Acids - HPLC

  • Performed by HPLC using the dedicated Biochrom 30 amino acid analyzer
  • The following can be measured: 1-Methylhistidine, 3-Methylhistidine, Alanine, Ammonia, Anserine, Arginine, Asparagine, Aspartic Acid, Carnosine, Citrulline, Cystathionine, Cysteine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocystine, Hydroxylysine-1, Hydroxylysine-2, Hydroxyproline, Isoleucine, Leucine, Lysine, Methionine, Norleucine, Ornithine, Phenylalanine, Phosphoethanolamine, Phosphoserine, Proline, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Urea, Valine, α-Aminoadipic acid, α-Aminoisobutyric acid, α-Amino-n-butyric acid, β-Alanine, γ-aminobutyric acid
  • An abbreviated "gluconeogenic" selection can be run at a lower cost: Alanine, Asparagine, Aspartic Acid, Glutamic Acid, Glutamine, Glycine, Serine, Threonine, Urea 
  • See the Sample Prep page for information about tissue prep.

Nucleotides - HPLC

  • An HPLC method was developed for the separation and quantification of AMP, ADP, ATP, ZMP and cAMP in tissue samples.
  • Separation is achieved within 30 minutes on a LC18-T chromatography column.
  • Detection is carried out using a Waters 490 UV detector.
  • These methods are unique and sensitive. Assays are carried out sporadically as the need arises.
  • See the Sample Prep page for information about tissue prep.

Luminex multiplex

  • This instrument allows for the x-map analysis of multiple analytes from a single small aliquot of sample.
  • See the Luminex page for more information.

Glucose - enzymatic 

Glucagon - ELISA

​Lipids

 

***This core is supported by NIH grants DK059637 (MMPC) and DK020593 (DRTC). Please acknowledge this in your publications.***