Hormones - RIA
- Used to measure ACTH, corticosterone, cortisol, c-peptide, ghrelin, glucagon, growth hormone, insulin, leptin, PYY, testosterone, T3 and T4
- Hormones are assayed by RIA using a double antibody procedure, allowing for the detection of minute concentrations of biological (hormones) or pharmacological substances in blood or other fluid samples using antigen/antibody reactions.
- The method provides a very sensitive, inexpensive method of measurement.
- Final analysis is accomplished by quantifying the bound radioactive counts with a Packard Gamma counter connected to a computerized data reduction station.
- These assays are high volume and their major benefit is in accuracy and cost savings.
- See the Assays page for vendor information
See the Sample Prep page for information about sample collection / required additives
Catecholamines - HPLC
- Plasma norepinephrine and epinephrine are routinely measured by HPLC via electrochemical detection. Dopamine is not measured.
- Plasma is absorbed onto alumina at a ph of 8.6, eluted with dilute perchloric acid, and auto-injected onto a c18 reversed-phase column.
- An internal standard (dehydroxylbenzylamine/DHBA) is included with each extraction to monitor recovery and aid in quantification.
- Results are quantified through a chromatography data station.
- This service is widely used and provides a precise and sensitive estimate of catecholamine levels in biologic tissues and fluids.
- Catecholamines are unstable. See the Sample Prep page for information about sample collection, required additives, and tissue prep.
Amino Acids - HPLC
- Performed by HPLC using the dedicated Biochrom 30 amino acid analyzer
- The following can be measured: 1-Methylhistidine, 3-Methylhistidine, Alanine, Ammonia, Anserine, Arginine, Asparagine, Aspartic Acid, Carnosine, Citrulline, Cystathionine, Cysteine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocystine, Hydroxylysine-1, Hydroxylysine-2, Hydroxyproline, Isoleucine, Leucine, Lysine, Methionine, Norleucine, Ornithine, Phenylalanine, Phosphoethanolamine, Phosphoserine, Proline, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Urea, Valine, α-Aminoadipic acid, α-Aminoisobutyric acid, α-Amino-n-butyric acid, β-Alanine, γ-aminobutyric acid
- An abbreviated "gluconeogenic" selection can be run at a lower cost: Alanine, Asparagine, Aspartic Acid, Glutamic Acid, Glutamine, Glycine, Serine, Threonine, Urea
See the Sample Prep page for information about tissue prep.
Nucleotides - HPLC
- An HPLC method was developed for the separation and quantification of AMP, ADP, ATP, ZMP and cAMP in tissue samples.
- Separation is achieved within 30 minutes on a LC18-T chromatography column.
- Detection is carried out using a Waters 490 UV detector.
- These methods are unique and sensitive. Assays are carried out sporadically as the need arises.
- See the Sample Prep page for information about tissue prep.
- This instrument allows for the x-map analysis of multiple analytes from a single small aliquot of sample.
- See the Luminex page for more information.
Glucose - enzymatic
- Glucose is measured by a glucose oxidation method using the Analox GM9 Glucose Analyzer.
Glucagon - ELISA
- In addition to the traditionally used RIA we now also offer analysis by the Mercodia Glucagon ELISA.
- This is a high quality two-site enzyme sandwich immunoassay for sensitive and specific determination of glucagon.
- There is little or no cross-reactivity to peptides oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP2 and GRPP. In contrast, ~20% of what is measured in the glucagon RIA is non-specific cross reacting material.
- Other advantages include low sample volume requirements (20 µl for duplicate analysis) and high sensitivity (2-180 pmol/L measurement range).
- See also:
Lipids are measured by the VUMC Lipid Core
***This core is supported by NIH grants DK059637 (MMPC) and DK020593 (DRTC). Please include an acknowledgment in your publications.***