ITR Discovery Panel

 Antibody TargetCell Type
CD8  Cytotoxic T cells
CD4  Helper T cells
CD20  B cells
FOXP3  Regulatory T cells
CD68  Macrophages
CD163  M2 Macrophages, monocytes
CD45  Hematopoietic cells
HLA-DR  APCs (MHC II)
Pan-Cytokeratin  Epithelial, tumor cells
10 CD31  Endothelial cells, 
  vascular structures
11 Ki67  Proliferating cells

The ITR Discovery Panel has been optimized on the following human FFPE tissues: 
• Breast
• Colon
• Lung
• Melanoma
• Prostate
• Renal Cell Carcinoma
• Lymph node
• Thymus
• Tonsil
If your tissue of interest is not included in this list, we will need to validate this panel on one of your tissue samples to ensure image quality is consistent with expected results before staining your entire cohort.

We stock the ITR Discovery Panel antibodies in our inventory, which allows us to offer this panel as a bundle. If you would like to add antibodies to this panel, we can do so at an additional cost, as we do have experience with several other biomarkers.

Please contact the ITR at kim.dahlman@vumc.org for further information detailing your PhenoCycler project!

Image Examples

Discovery Panel in lung, melanoma, and prostate

Discovery Panel in breast cancer and colon tissue