Parnell JM, Fazili I, Bloch SC, Lacy DB, Garcia-Lopez VA, Bernard R, Skaar EP, Edwards KM, Nicholson MR. Two-Step Testing for Clostridioides Difficile is Inadequate in Differentiating Infection From Colonization in Children. Journal of pediatric gastroenterology and nutrition. 2020 Sep 9. PMID: 32925555 [PubMed]
Recent Infectious Disease Society of America guidelines recommend multi-step testing algorithms to diagnose Clostridioides difficile infection (CDI), including a combination of nucleic acid amplification-based testing (NAAT) and toxin enzyme immunoassay (EIA). However, the use of these algorithms in children, including the ability to differentiate between C. difficile colonization and CDI, has not been evaluated.