Sort up to 6 populations using the 3 cell sorters in our facility as well as 1 BSL2+ cell sorter. 3 BD FACS Aria III cell sorters and 1 Cytek CS Cell Sorter. All sorters are capable of sorting at a high rate and excellent purity and recovery. Each sorter is enclosed in biosafety cabinets
BSL2+ Cell Sorter is available for live human infectious samples. Please contact VUMC Flow Core as well as OCRS before booking for proper containment.
Laser lines for each sorter:
FACS Aria III (BioBubble): 405nm, 488nm, 561nm, 633nm
FACS Aria III (BakerHood): 405nm, 488nm, 561nm, 633nm
FACS Aria III (BSL2+): 405nm, 488nm, 561nm, 633nm
Cytek CS Sorter: 355nm, 405nm, 488nm, 561nm, 633nm
Sorting Capabilities:
- Single-cell sorting into 96 or 384 well plates
- Nozzle sizes: 70um, 100um, 130um
- Bulk sorting into 1.5 Eppendorf tubes, 5mL round bottom tubes, 15mL conicals, 6-well plates, 24-well plates,
Below are links to our standard filter configurations for each instrument. The filters do have the ability to change based on your fluorochromes spectra, reach out to any of our assistants if you are unsure about a specific panel.
Aria III Baker Hood Configuration
Aria III Bio Bubble Configuration
