Enkhbaatar P, Nelson C, Salsbury JR, Carmical JR, Torres KE, Herndon D, Prough DS, Luan L, Sherwood ER. Comparison of Gene Expression by Sheep and Human Blood Stimulated with the TLR4 Agonists Lipopolysaccharide and Monophosphoryl Lipid A. PloS one. 10(10). e0144345. PMID: 26640957 [PubMed] PMCID: PMC4671644
Animal models that mimic human biology are important for successful translation of basic science discoveries into the clinical practice. Recent studies in rodents have demonstrated the efficacy of TLR4 agonists as immunomodulators in models of infection. However, rodent models have been criticized for not mimicking important characteristics of the human immune response to microbial products. The goal of this study was to compare genomic responses of human and sheep blood to the TLR4 agonists lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA).