Resistance-conferring mutations on whole-genome sequencing of fluoroquinolone-resistant and -susceptible M. tuberculosis isolates: a proposed threshold for identifying resistance.

Abstract

Fluoroquinolone resistance in Mycobacterium tuberculosis is conferred by DNA gyrase mutations, but not all fluoroquinolone-resistant M. tuberculosis isolates have mutations detected. The optimal allele frequency threshold to identify resistance-conferring mutations by whole-genome sequencing is unknown.