Publications

Wilson MH, Gottschalk S. Expect the unexpected: piggyBac and lymphoma. Blood. 2021 Oct 21;138(16):1379-1380. doi: 10.1182/blood.2021012349. PMID: 34673949.

Beckermann TM, Luo W, Wilson CM, Veach RA, Wilson MH. Cognate restriction of transposition by piggyBac-like proteins. Nucleic Acids Res. 2021 Jul 7:gkab578. doi: 10.1093/nar/gkab578. Epub ahead of print. PMID: 34232995.

Reinfeld BI, Madden MZ, Wolf MM, Chytil A, Bader JE, Patterson AR, Sugiura A, Cohen AS, Ali A, Do BT, Muir A, Lewis CA, Hongo RA, Young KL, Brown RE, Todd VM, Huffstater T, Abraham A, O'Neil RT, Wilson MH, Xin F, Tantawy MN, Merryman WD, Johnson RW, Williams CS, Mason EF, Mason FM, Beckermann KE, Vander Heiden MG, Manning HC, Rathmell JC, Rathmell WK. Cell-programmed nutrient partitioning in the tumour microenvironment. Nature. 2021 Apr 7. doi: 10.1038/s41586-021-03442-1. Epub ahead of print. PMID: 33828302.

Woodard LE, Williams FM, Jarrett IC, Wilson MH. Functional analysis of the catalytic triad of the hAT-family transposase TcBuster. Plasmid. 2021 Mar;114:102554. doi: 10.1016/j.plasmid.2021.102554. Epub 2021 Jan 18. PMID: 33476638; PMCID: PMC7946762.

Reinfeld BI, Madden MZ, Wolf MM, Chytil A, Bader JE, Patterson AR, Cohen AS, Ali A, Do BT, Muir A, Lewis CA, Hongo RA, Young KL, Brown RE, Todd VM, Huffstater T, Abraham A, O’Neil RT, Wilson MH, Xin F, Tantawy MN, Merryman WD, Johnson RW, Williams CS, Mason EF, Mason FM, Beckermann KE, Vander Heiden MG, Manning HC, Rathmell JC, Rathmell WK. Cell Programmed Nutrient Partitioning in the Tumor Microenvironment. bioRxiv 2020.08.10.238428.

Chiusa M, Hu W, Zienkiewicz J, Chen X, Zhang MZ, Harris RC, Vanacore RM, Bentz JA, Remuzzi G, Benigni A, Fogo AB, Luo W, Mili S, Wilson MH, Zent R, Hawiger J, Pozzi A. EGF receptor-mediated FUS phosphorylation promotes its nuclear translocation and fibrotic signaling. Journal of Cell Biology. 2020, 219(9):e202001120.

Chen Q, Luo W, Veach RA, Hickman AB, Wilson MH, Dyda F. Structural basis of seamless excision and specific targeting by piggyBac transposase. Nat Commun. 2020;11(1):3446. Published 2020 Jul 10. doi:10.1038/s41467-020-17128-1

Love HD, Evans RC, Humes HD, Roy S, Zent R, Harris R, Wilson MH, Fissell WH. Metformin and Inhibition of TGF-β Stimulate In Vitro Transport in Primary Renal Tubule Cells. Tissue Eng Part A. 2020 Apr 20;. doi: 10.1089/ten.TEA.2019.0294. [Epub ahead of print] PubMed PMID: 32312181.

Beckermann TM, Welch RC, Williams FM, Mortlock DP, Sha F, Ikizler TA, Woodard LE, Wilson MH. CRISPR/Cas9 engineering of albino cystinuria Type A mice. Genesis. 2020 Feb 20:e23357. doi: 10.1002/dvg.23357. [Epub ahead of print] PMID: 32078250

Wilson MH, Veach RA, Luo W, Welch RC, Roy S, Fissell WH. Genome Engineering Renal Epithelial Cells for Enhanced Volume Transport Function. Cell Mol Bioeng. 2019 Sep 25;13(1):17-26. doi: 10.1007/s12195-019-00601-3. eCollection 2020 Feb. PMID: 32030105

Woodard LE, Welch RC, Veach RA, Beckermann TM, Sha F, Weinman EJ, Ikizler TA, Tischfield JA, Sahota A, Wilson MH. Metabolic consequences of cystinuria. BMC Nephrol. 2019 Jun 20;20(1):227. doi: 10.1186/s12882-019-1417-8. PubMed PMID: 31221135; PubMed Central PMCID: PMC6585015.

Vanslambrouck JM, Woodard LE, Suhaimi N, Williams FM, Howden SE, Wilson SB, Lonsdale A, Er PX, Li J, Maksimovic J, Oshlack A, Wilson MH, Little MH. Direct reprogramming to human nephron progenitor-like cells using inducible piggyBac transposon expression of SNAI2-EYA1-SIX1. Kidney Int. 2019 May;95(5):1153-1166. doi: 10.1016/j.kint.2018.11.041. Epub 2019 Feb 28. PubMed PMID: 30827514; PubMed Central PMCID: PMC6478531.

Veach RA, Wilson MH. CRISPR/Cas9 engineering of a KIM-1 reporter human proximal tubule cell line. PLoS One. 2018;13(9):e0204487. doi: 10.1371/journal.pone.0204487. eCollection 2018. PubMed PMID: 30260998; PubMed Central PMCID: PMC6160069.

Hamada M, Nishio N, Okuno Y, Suzuki S, Kawashima N, Muramatsu H, Tsubota S, Wilson MH, Morita D, Kataoka S, Ichikawa D, Murakami N, Taniguchi R, Suzuki K, Kojima D, Sekiya Y, Nishikawa E, Narita A, Hama A, Kojima S, Nakazawa Y, Takahashi Y. Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR. EBioMedicine. 2018 Aug;34:18-26. doi: 10.1016/j.ebiom.2018.07.008. Epub 2018 Aug 3. PubMed PMID: 30082227; PubMed Central PMCID: PMC6116345.

Wilson MH. Consider Changing the Horse for Your CAR-T?. Mol Ther. 2018 Aug 1;26(8):1873-1874. doi: 10.1016/j.ymthe.2018.06.018. Epub 2018 Jun 30. PubMed PMID: 30078438; PubMed Central PMCID: PMC6094485.

O'Neil RT, Saha S, Veach RA, Welch RC, Woodard LE, Rooney CM, Wilson MH. Transposon-modified antigen-specific T lymphocytes for sustained therapeutic protein delivery in vivo. Nat Commun. 2018 Apr 10;9(1):1325. doi: 10.1038/s41467-018-03787-8. PubMed PMID: 29636469; PubMed Central PMCID: PMC5893599.

Morita D, Nishio N, Saito S, Tanaka M, Kawashima N, Okuno Y, Suzuki S, Matsuda K, Maeda Y, Wilson MH, Dotti G, Rooney CM, Takahashi Y, Nakazawa Y. Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells. Mol Ther Methods Clin Dev. 2018 Mar 16;8:131-140. doi: 10.1016/j.omtm.2017.12.003. eCollection 2018 Mar 16. PubMed PMID: 29687032; PubMed Central PMCID: PMC5907825.

Woodard LE, Welch RC, Williams FM, Luo W, Cheng J, Wilson MH. Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney. J Vis Exp. 2018 Jan 8;(131). doi: 10.3791/56324. PubMed PMID: 29364221; PubMed Central PMCID: PMC5907682.

Woodard LE, Galvan DL, Wilson MH. Site-Directed Genome Modification with Engineered Zinc Finger Proteins . In: Synthetic Biology: Parts, Devices and Applications. Smolke C, editor. Weinheim, Germany: Wiley-VCH; 2018. Chapter 3; p.33-48.

Luo W, Galvan DL, Woodard LE, Dorset D, Levy S, Wilson MH. Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells. Nucleic Acids Res. 2017 Aug 21;45(14):8411-8422. doi: 10.1093/nar/gkx572. PubMed PMID: 28666380; PubMed Central PMCID: PMC5737283.

Woodard LE, Cheng J, Welch RC, Williams FM, Luo W, Gewin LS, Wilson MH. Kidney-specific transposon-mediated gene transfer in vivo. Sci Rep. 2017 Mar 20;7:44904. doi: 10.1038/srep44904. PubMed PMID: 28317878; PubMed Central PMCID: PMC5357952.

Woodard LE, Downes LM, Lee YC, Kaja A, Terefe ES, Wilson MH. Temporal self-regulation of transposition through host-independent transposase rodlet formation. Nucleic Acids Res. 2017 Jan 9;45(1):353-366. doi: 10.1093/nar/gkw1115. Epub 2016 Nov 28. PubMed PMID: 27899587; PubMed Central PMCID: PMC5224482.

Held EP, Iglesia EA, Johnson AS, Fang JY, Wilson MH, Abel TW, Lake W, Siew ED. Neurosarcoidosis Presenting as Aseptic Meningitis in an Immunosuppressed Renal Transplant Recipient. Transplantation. 2016 Oct;100(10):e96-e100. doi: 10.1097/TP.0000000000001074. PubMed PMID: 26863472.

Nakazawa Y, Matsuda K, Kurata T, Sueki A, Tanaka M, Sakashita K, Imai C, Wilson MH, Koike K. Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34(+) cells of juvenile myelomonocytic leukemia. J Hematol Oncol. 2016 Mar 16;9:27. doi: 10.1186/s13045-016-0256-3. PubMed PMID: 26983639; PubMed Central PMCID: PMC4793548.

Galvan DL, O'Neil RT, Foster AE, Huye L, Bear A, Rooney CM, Wilson MH. Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model. PLoS One. 2015;10(10):e0140744. doi: 10.1371/journal.pone.0140744. eCollection 2015. PubMed PMID: 26473608; PubMed Central PMCID: PMC4608718.

Woodard LE, Wilson MH. piggyBac-ing models and new therapeutic strategies. Trends Biotechnol. 2015 Sep;33(9):525-33. doi: 10.1016/j.tibtech.2015.06.009. Epub 2015 Jul 23. Review. PubMed PMID: 26211958; PubMed Central PMCID: PMC4663986.

Liang M, Woodard LE, Liang A, Luo J, Wilson MH, Mitch WE, Cheng J. Protective role of insulin-like growth factor-1 receptor in endothelial cells against unilateral ureteral obstruction-induced renal fibrosis. Am J Pathol. 2015 May;185(5):1234-50. doi: 10.1016/j.ajpath.2015.01.027. Epub 2015 Mar 14. PubMed PMID: 25783760; PubMed Central PMCID: PMC4419212.

Saha S, Woodard LE, Charron EM, Welch RC, Rooney CM, Wilson MH. Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells. Nucleic Acids Res. 2015 Feb 18;43(3):1770-82. doi: 10.1093/nar/gkv017. Epub 2015 Jan 20. PubMed PMID: 25605795; PubMed Central PMCID: PMC4330379.

Saito S, Nakazawa Y, Sueki A, Matsuda K, Tanaka M, Yanagisawa R, Maeda Y, Sato Y, Okabe S, Inukai T, Sugita K, Wilson MH, Rooney CM, Koike K. Anti-leukemic potency of piggyBac-mediated CD19-specific T cells against refractory Philadelphia chromosome-positive acute lymphoblastic leukemia. Cytotherapy. 2014 Sep;16(9):1257-69. doi: 10.1016/j.jcyt.2014.05.022. PubMed PMID: 25108652; PubMed Central PMCID: PMC4948190.

Galvan DL, Kettlun CS, Wilson MH. Targeting piggyBac transposon integrations in the human genome. Methods Mol Biol. 2014;1114:143-61. doi: 10.1007/978-1-62703-761-7_9. PubMed PMID: 24557901.

Doherty JE, Woodard LE, Bear AS, Foster AE, Wilson MH. An adaptable system for improving transposon-based gene expression in vivo via transient transgene repression. FASEB J. 2013 Sep;27(9):3753-62. doi: 10.1096/fj.13-232090. Epub 2013 Jun 10. PubMed PMID: 23752206; PubMed Central PMCID: PMC3752539.

Saha S, Wilson MH. Transposons for Non-Viral Gene Transfer . In: Gene Therapy - Tools and Potential Applications. Molina FM, editor. www.intechopen.com: InTech; 2013. p.269-285.

Nakazawa Y, Saha S, Galvan DL, Huye L, Rollins L, Rooney CM, Wilson MH. Evaluation of long-term transgene expression in piggyBac-modified human T lymphocytes. J Immunother. 2013 Jan;36(1):3-10. doi: 10.1097/CJI.0b013e3182791234. PubMed PMID: 23211626; PubMed Central PMCID: PMC3521868.

Liang A, Wang Y, Woodard LE, Wilson MH, Sharma R, Awasthi YC, Du J, Mitch WE, Cheng J. Loss of glutathione S-transferase A4 accelerates obstruction-induced tubule damage and renal fibrosis. J Pathol. 2012 Dec;228(4):448-58. doi: 10.1002/path.4067. Epub 2012 Jul 26. PubMed PMID: 22711583; PubMed Central PMCID: PMC3760987.

Woodard LE, Li X, Malani N, Kaja A, Hice RH, Atkinson PW, Bushman FD, Craig NL, Wilson MH. Comparative analysis of the recently discovered hAT transposon TcBuster in human cells. PLoS One. 2012;7(11):e42666. doi: 10.1371/journal.pone.0042666. Epub 2012 Nov 15. PubMed PMID: 23166581; PubMed Central PMCID: PMC3499496.

Saha S, Nakazawa Y, Huye LE, Doherty JE, Galvan DL, Rooney CM, Wilson MH. piggyBac transposon system modification of primary human T cells. J Vis Exp. 2012 Nov 5;(69):e4235. doi: 10.3791/4235. PubMed PMID: 23149543; PubMed Central PMCID: PMC3514050.

Doherty JE, Huye LE, Yusa K, Zhou L, Craig NL, Wilson MH. Hyperactive piggyBac gene transfer in human cells and in vivo. Hum Gene Ther. 2012 Mar;23(3):311-20. doi: 10.1089/hum.2011.138. Epub 2011 Dec 14. PubMed PMID: 21992617; PubMed Central PMCID: PMC3300075.

Nakazawa Y, Huye LE, Salsman VS, Leen AM, Ahmed N, Rollins L, Dotti G, Gottschalk SM, Wilson MH, Rooney CM. PiggyBac-mediated cancer immunotherapy using EBV-specific cytotoxic T-cells expressing HER2-specific chimeric antigen receptor. Mol Ther. 2011 Dec;19(12):2133-43. doi: 10.1038/mt.2011.131. Epub 2011 Jul 19. PubMed PMID: 21772253; PubMed Central PMCID: PMC3242651.

Huye LE, Nakazawa Y, Patel MP, Yvon E, Sun J, Savoldo B, Wilson MH, Dotti G, Rooney CM. Combining mTor inhibitors with rapamycin-resistant T cells: a two-pronged approach to tumor elimination. Mol Ther. 2011 Dec;19(12):2239-48. doi: 10.1038/mt.2011.179. Epub 2011 Aug 30. PubMed PMID: 21878902; PubMed Central PMCID: PMC3242659.

Kettlun C, Galvan DL, George AL Jr, Kaja A, Wilson MH. Manipulating piggyBac transposon chromosomal integration site selection in human cells. Mol Ther. 2011 Sep;19(9):1636-44. doi: 10.1038/mt.2011.129. Epub 2011 Jul 5. PubMed PMID: 21730970; PubMed Central PMCID: PMC3182353.

Wang J, Wang Y, Long J, Chang BH, Wilson MH, Overbeek P, Danesh FR. Tamoxifen-inducible podocyte-specific iCre recombinase transgenic mouse provides a simple approach for modulation of podocytes in vivo. Genesis. 2010 Jul;48(7):446-51. doi: 10.1002/dvg.20635. PubMed PMID: 20641128; PubMed Central PMCID: PMC3014324.

Manuri PV, Wilson MH, Maiti SN, Mi T, Singh H, Olivares S, Dawson MJ, Huls H, Lee DA, Rao PH, Kaminski JM, Nakazawa Y, Gottschalk S, Kebriaei P, Shpall EJ, Champlin RE, Cooper LJ. piggyBac transposon/transposase system to generate CD19-specific T cells for the treatment of B-lineage malignancies. Hum Gene Ther. 2010 Apr;21(4):427-37. doi: 10.1089/hum.2009.114. PubMed PMID: 19905893; PubMed Central PMCID: PMC2938363.

Kahlig KM, Saridey SK, Kaja A, Daniels MA, George AL Jr, Wilson MH. Multiplexed transposon-mediated stable gene transfer in human cells. Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1343-8. doi: 10.1073/pnas.0910383107. Epub 2010 Jan 6. PubMed PMID: 20080581; PubMed Central PMCID: PMC2824351.

Wilson MH, George AL Jr. Designing and testing chimeric zinc finger transposases. Methods Mol Biol. 2010;649:353-63. doi: 10.1007/978-1-60761-753-2_22. PubMed PMID: 20680846.

Saridey SK, Liu L, Doherty JE, Kaja A, Galvan DL, Fletcher BS, Wilson MH. PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer. Mol Ther. 2009 Dec;17(12):2115-20. doi: 10.1038/mt.2009.234. Epub 2009 Oct 6. PubMed PMID: 19809403; PubMed Central PMCID: PMC2814386.

Nakazawa Y, Huye LE, Dotti G, Foster AE, Vera JF, Manuri PR, June CH, Rooney CM, Wilson MH. Optimization of the PiggyBac transposon system for the sustained genetic modification of human T lymphocytes. J Immunother. 2009 Oct;32(8):826-36. doi: 10.1097/CJI.0b013e3181ad762b. PubMed PMID: 19752751; PubMed Central PMCID: PMC2796278.

Galvan DL, Nakazawa Y, Kaja A, Kettlun C, Cooper LJ, Rooney CM, Wilson MH. Genome-wide mapping of PiggyBac transposon integrations in primary human T cells. J Immunother. 2009 Oct;32(8):837-44. doi: 10.1097/CJI.0b013e3181b2914c. PubMed PMID: 19752750; PubMed Central PMCID: PMC2796288.

Wilson MH, Coates CJ, George AL Jr. PiggyBac transposon-mediated gene transfer in human cells. Mol Ther. 2007 Jan;15(1):139-45. doi: 10.1038/sj.mt.6300028. PubMed PMID: 17164785.

Tan CM, Wilson MH, MacMillan LB, Kobilka BK, Limbird LE. Heterozygous alpha 2A-adrenergic receptor mice unveil unique therapeutic benefits of partial agonists. Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12471-6. doi: 10.1073/pnas.122368499. Epub 2002 Aug 30. PubMed PMID: 12205290; PubMed Central PMCID: PMC129469.

Wilson MH, Highfield HA, Limbird LE. The role of a conserved inter-transmembrane domain interface in regulating alpha(2a)-adrenergic receptor conformational stability and cell-surface turnover. Mol Pharmacol. 2001 Apr;59(4):929-38. PubMed PMID: 11259639.

Wilson MH, Limbird LE. Mechanisms regulating the cell surface residence time of the alpha 2A-adrenergic receptor. Biochemistry. 2000 Feb 1;39(4):693-700. PubMed PMID: 10651634.

Wilson MH, Puranam RS, Ottman R, Gilliam C, Limbird LE, George AL Jr, McNamara JO. Evaluation of the alpha(2A)-adrenergic receptor gene in a heritable form of temporal lobe epilepsy. Neurology. 1998 Dec;51(6):1730-1. PubMed PMID: 9855534.