The Immunology Core provides a wide-range of support and assays to assist investigators in their research with a primary interest in assessing cellular immune responses during clinical trials. We strive to assist investigators with sample processing, storage, shipping, documentation, assay design, assay development, and evaluation of immune responses. Our dedicated staff can provide consultation to investigators on the types of immune assays to choose for clinical trial monitoring and data interpretation. It is the goal of the core to monitor immune responses during clinical trials using the latest advances in technology and provide practical applications of this technology to evaluating immune responses. We continue to develop new immune monitoring technology with industry and academic collaborators. The immunology core has extensive experience in assay development and optimization and will assist in the selection of appropriate assays and antigens during the protocol/developmental phase of a study or trial. A brief description of assays are listed below, however; each study design will have unique features that require careful consideration and development and optimization prior to testing samples. Thus, specific assay conditions are not described in detail. 

 

The Immunology Core provides a range of immune monitoring techniques and assays including:

  • Consultation on experimental and clinical trial design involving immune monitoring
  • Individual written protocol and budget preparation
  • Processing and storage of samples (Peripheral Blood, Serum, Plasma, etc.)
  • Protein Measurements - Multiplex 96-well plate based assays (Cytometric Bead Array and Meso Scale Discovery platforms)
  • Elispot Assays
  • Phenotypic analysis with flow cytometry
  • Multifunctional/Polychromatic flow cytometry (7-14 color)
  • Intracellular staining (cytokines, granzymes, phosphorylation, etc.)
  • Cell proliferation assays
  • Tetramer/pentamer staininng
  • Influenza HAI
  • Pertussis ELISA
  • Commercial ELISA
  • Data analysis, management and reporting
  • Generation and maintenance of viability and cell recovery data

For more information,

Contact James Crowe 

james.crowe@vanderbilt.edu
615-343-8064 (Office)

Or visit http://www.mc.vanderbilt.edu/root/vumc.php?site=immunocore

Immunology Core