VVC Seminar Series

Join us Monday, December 3rd from 11AM - Noon in 202 Light Hall!

 

"Structural and Functional Characterization of Broadly Reactive Antibodies Against Filoviruses Induced by Multivalent Immunization"

 

Gilad Adi Ofek, PhD

 

Department of Cell Biology and Molecular Genetics, Institute for Bioscience and Biotechnology Research
University of Maryland College Park, Rockville, MD 20850

 

During the 2014 outbreak of Ebola virus in West Africa, most immunotherapeutics and vaccines in late stage development were monospecific and lacked efficacy against other filoviruses that previously emerged. Efforts were thus initiated to isolate and characterize antibodies with broader efficacy. Such antibodies not only help expand the clinical toolkit against diverse future outbreaks, but also define sites of broad immune vulnerability for vaccine development. Several recent studies have shown that broadly-reactive neutralizing antibodies against ebolaviruses can be induced both in natural infection, as well as through animal immunizations. In this talk, I will discuss my lab’s efforts to structurally and functionally characterize broadly-reactive antibodies isolated from immunized nonhuman primates. Our approach has been based on immunization with multivalent cocktails of recombinant filovirus glycoproteins and virus like particles. Of particular recent focus has been a near pan-ebolavirus reactive antibody, CA45, that was induced by such immunizations. Our crystal structure of CA45 bound to the Ebola surface glycoprotein reveals that it targets a highly conserved site on the fusion loop stem of the glycoprotein, one that overlaps a previously defined pan-reactive inhibitor binding cavity. Through structural and functional analyses of antibodies elicited by immunization, we are defining novel molecular determinants on filoviruses that confer broad antibody recognition and neutralization – determinants that are being applied for further development of related therapeutics and vaccines.

2017 Vanderbilt Symposium on Modeling Immunity

Thursday, April 27

1:00 pm - 4:00 pm

1220 MRB III

Register by April 7 to guarantee lunch! 
Reception to follow in MRB III lobby.

Speakers:

Sarel Fleishman, Ph.D.

Senior Scientist, Department of Biomolecular Sciences; Martha S. Sagon Career Development Chair, Weizmann Institute of Science

Frank DiMaio, Ph.D.

Assistant Professor, Department of Biochemistry, University of Washington

Steven Kleinstein, Ph.D.

Associate Professor of Pathology, Yale School of Medicine 

Andrew Ward, Ph.D.

Associate Professor, Department of Integrative Structural and Computational Biology, The Scripps Research Institute

Jens Meiler, Ph.D.

Professor, Department of Chemistry and Pharmacology, Vanderbilt University

 

2016 Vanderbilt Symposium on Modeling Immunity

Thursday, April 28

9:00 am – 12:30 pm

202 Light Hall

Plenary Speaker:

David Baker, Ph.D.

Professor, Department of Biochemistry; Head of the Institute for Protein Design, University of Washington

Featured Speakers:

Ian Wilson, Ph.D.

Hansen Professor of Structural Biology; Chair, Department of Integrative Structural and Computational Biology, The Scripps Institute

Andrew Ward, Ph.D.

Associate Professor, Department of Integrative Structural and Computational Biology, The Scripps Institute

William Schief, Ph.D.

Professor, Department of Immunology and Microbial Science, The Scripps Institute

Jens Meiler, Ph.D.

Associate Professor, Department of Chemistry and Pharmacology, Vanderbilt University

James E. Crowe, Jr., M.D.

Professor, Department of Pediatrics and Pathology, Microbiology, and Immunology; Director, Vanderbilt Vaccine Center, Vanderbilt University

Ebola Outbreak Symposium

Posted in

Watch the full-length symposium here:

2015 Ebola Symposium

Vanderbilt Vaccine Center 

Presents

 “Ebola Outbreak in Nigeria 2014:
A First Hand Report from Survivors and Healthcare Providers”

 Featuring

Benjamin Nnamdi Ohiaeri, M.D.

Founder and Chief Medical Director of First Consultants Medical Center Limited

 

 Ada Igonoh, M.B.B.S.
Morris Ibeawuchi, M.B.B.S.
Ige Adewale Adejoro, M.B.B.S.

Ebola Survivors; Physicians, First Consultants Medical Center Limited

Hosted by

 James E. Crowe Jr., MD
Ann Scott Carell Chair of Pediatrics and Pathology, Microbiology, and Immunology

 2:00 – 3:30
Wednesday, September 16th, 2015
208 Light Hall

Reception to Follow in Light Hall Lobby

 

The founder and Medical Director of First Consultants Medical Center in Lagos, Nigeria, 
will be on campus to discuss how his hospital contained the Ebola outbreak in Lagos. 
Three physicians from this hospital who survived Ebola are also traveling to Nashville to discuss their experience.