Join us Friday, March 15th at 3 PM in 202 Light Hall!
Join us Monday, December 3rd from 11AM - Noon in 202 Light Hall!
"Structural and Functional Characterization of Broadly Reactive Antibodies Against Filoviruses Induced by Multivalent Immunization"
Gilad Adi Ofek, PhD
Department of Cell Biology and Molecular Genetics, Institute for Bioscience and Biotechnology Research
University of Maryland College Park, Rockville, MD 20850
During the 2014 outbreak of Ebola virus in West Africa, most immunotherapeutics and vaccines in late stage development were monospecific and lacked efficacy against other filoviruses that previously emerged. Efforts were thus initiated to isolate and characterize antibodies with broader efficacy. Such antibodies not only help expand the clinical toolkit against diverse future outbreaks, but also define sites of broad immune vulnerability for vaccine development. Several recent studies have shown that broadly-reactive neutralizing antibodies against ebolaviruses can be induced both in natural infection, as well as through animal immunizations. In this talk, I will discuss my lab’s efforts to structurally and functionally characterize broadly-reactive antibodies isolated from immunized nonhuman primates. Our approach has been based on immunization with multivalent cocktails of recombinant filovirus glycoproteins and virus like particles. Of particular recent focus has been a near pan-ebolavirus reactive antibody, CA45, that was induced by such immunizations. Our crystal structure of CA45 bound to the Ebola surface glycoprotein reveals that it targets a highly conserved site on the fusion loop stem of the glycoprotein, one that overlaps a previously defined pan-reactive inhibitor binding cavity. Through structural and functional analyses of antibodies elicited by immunization, we are defining novel molecular determinants on filoviruses that confer broad antibody recognition and neutralization – determinants that are being applied for further development of related therapeutics and vaccines.
Thursday, April 27
1:00 pm - 4:00 pm
1220 MRB III
Register by April 7 to guarantee lunch!
Reception to follow in MRB III lobby.
Sarel Fleishman, Ph.D.
Senior Scientist, Department of Biomolecular Sciences; Martha S. Sagon Career Development Chair, Weizmann Institute of Science
Frank DiMaio, Ph.D.
Assistant Professor, Department of Biochemistry, University of Washington
Steven Kleinstein, Ph.D.
Associate Professor of Pathology, Yale School of Medicine
Andrew Ward, Ph.D.
Associate Professor, Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Jens Meiler, Ph.D.
Professor, Department of Chemistry and Pharmacology, Vanderbilt University
Thursday, April 28
9:00 am – 12:30 pm
202 Light Hall
David Baker, Ph.D.
Professor, Department of Biochemistry; Head of the Institute for Protein Design, University of Washington
Ian Wilson, Ph.D.
Hansen Professor of Structural Biology; Chair, Department of Integrative Structural and Computational Biology, The Scripps Institute
Andrew Ward, Ph.D.
Associate Professor, Department of Integrative Structural and Computational Biology, The Scripps Institute
William Schief, Ph.D.
Professor, Department of Immunology and Microbial Science, The Scripps Institute
Jens Meiler, Ph.D.
Associate Professor, Department of Chemistry and Pharmacology, Vanderbilt University
James E. Crowe, Jr., M.D.
Professor, Department of Pediatrics and Pathology, Microbiology, and Immunology; Director, Vanderbilt Vaccine Center, Vanderbilt University
Watch the full-length symposium here:
Vanderbilt Vaccine Center
“Ebola Outbreak in Nigeria 2014:
A First Hand Report from Survivors and Healthcare Providers”
Benjamin Nnamdi Ohiaeri, M.D.
Founder and Chief Medical Director of First Consultants Medical Center Limited
Ada Igonoh, M.B.B.S.
Morris Ibeawuchi, M.B.B.S.
Ige Adewale Adejoro, M.B.B.S.
Ebola Survivors; Physicians, First Consultants Medical Center Limited
James E. Crowe Jr., MD
Ann Scott Carell Chair of Pediatrics and Pathology, Microbiology, and Immunology
2:00 – 3:30
Wednesday, September 16th, 2015
208 Light Hall
Reception to Follow in Light Hall Lobby
The founder and Medical Director of First Consultants Medical Center in Lagos, Nigeria,
will be on campus to discuss how his hospital contained the Ebola outbreak in Lagos.
Three physicians from this hospital who survived Ebola are also traveling to Nashville to discuss their experience.