The Vanderbilt Analytical Services Core facilitates diabetes, endocrine, and metabolic research across a range of species. Sensitive, reproducible, and inexpensive analysis of hormones, lipids, amino acids, stable isotopes, and markers of oxidative stress and inflammation is provided. Investigators are given access to assays which are not readily available, assistance with method development, training, technical support, and access to research space and personnel.
Subcore Director: Dale Edgerton, Ph.D. (dale.edgerton@vanderbilt.edu)
Facility Manager: Pat Donahue (patrick.donahue@vumc.org)
Administrative Officer: Alisa Escue (alisa.escue@vumc.org)
RIA / HPLC / Luminex / Enzymatic / ELISA assays:
- Radioimmunoassays (RIA): ACTH, corticosterone, cortisol, c-peptide, ghrelin, glucagon, insulin, leptin, PYY, testosterone, T3 and T4
- High Performance Liquid Chromatography (HPLC): amino acids and catecholamines (epinephrine & norepinephrine)
- Luminex multiplex: includes human, mouse and canine adipokine, cytokine/chemokine, metabolic hormones, IGF, immunology/inflammation, and pituitary panels
- Enzymatic: glucose
- ELISA: glucagon
* For inquiries, please contact the hormone core staff at (615) 936-1626 or email Dale Edgerton
LIPID ASSAYS:
- Enzymatic Lipid Profiles (plasma):
- cholesterol, triglycerides, HDL cholesterol, free fatty acids
- Gas Chromatography (GC) Lipid Analysis (tissue, cell culture, plasma):
- phospholipids, diglycerides, free fatty acids, triglycerides, cholesterol esters (includes the fatty acid profile and quantitation of each lipid class)
- total cholesterol, unesterified cholesterol (quantitation only)
- Fast Protein Liquid Chromatography (FPLC) Lipoprotein Separation (plasma):
- lipoprotein profile (includes plasma total cholesterol and triglycerides)
* For inquiries, please contact the hormone core staff at (615) 343-2645 or email Carla Harris
Metabolomics:
- Small molecule biomarker analysis with bioinformatic and statistical analysis and biochemical and biological interpretation of results.
- The Hormone Core has a contract with Metabolon to provide metabolomics analysis. This service is only available to internal (VU/VUMC) investigators.
* For inquiries, please email Alisa Escue
***This core is supported by NIH grant DK020593 (DRTC). Please acknowledge this in your publications.***
