Completed Antibody Panels

Panel size: 14 antibodies
   
Validated tissue type(s): Fresh frozen human head and neck squamous cell carcinoma (HNSCC) tissue
   
1  CD45
2  CD4
3  CD8
4  HLA-DR
5  Pan-Cytokeratin
6  Ki67
7  CD11c
8  CD31
9  CD19
10  CD69
11  CD104
12  PD-1 (CD279)
13  CD38
14  CD3
Panel size: 14 antibodies
   
Validated tissue type(s): FFPE human renal cell carcinoma (RCC)
  FFPE human tonsil
1  CD45
2  CD4
3  CD8
4  HLA-DR
5  Pan-Cytokeratin
6  Ki67
7  CD11c
8  CD31
9  CD20
10  CD68
11  CD107a
12  Podoplanin
13  TFAM
14  CD3e
  Panel size: 24 antibodies
     
  Validated tissue type(s): FFPE human breast tissue (normal, DCIS, invasive carcinoma)
     FFPE human tonsil
1  CD45 15  HLA-A
2  CD4 16  CD56
3  CD8 17  CD45RO
4  HLA-DR 18  GATA3
5  Pan-Cytokeratin 19  ERα
6  Ki67 20  Keratin8/18
7  CD11c 21  Keratin5
8  PD-L1 22  HER2
9  CD20 23  PR
10  CD68 24  AR
11  CD107a
12  FOXP3
13  Granzyme B
14  CD163
Antibodies labeled in green are not available from Akoya Biosciences and were conjugated to barcodes by the ITR.

The ITR has successfully used the above panels on the indicated tissue types.

Please note that antibody clones used on fresh frozen tissue are different from those used on FFPE tissue, so panels developed on one sample type may not be used on the other. If you want to use the above panels on tissue types not listed above, we will need to optimize the panel for your tissue (e.g. re-titrate antibodies, incorporate a tissue autofluorescence quenching protocol).

We are looking forward to taking on new projects and developing more panels in the future.

 

If you would like to see Akoya Biosciences’ current catalog of pre-conjugated antibodies, please click the following link and view the section labeled “PhenoCycler Antibodies:”

https://www.akoyabio.com/phenocycler/assays/

We also provide a conjugation service for antibodies against targets which are not listed in the catalog. The major requirement for conjugation is that the antibody is monoclonal. Ideally, the antibody should also be compatible with immunohistochemistry (IHC). To validate the antibody, we will use the unconjugated antibody in chromogenic IHC and compare its staining pattern to the conjugated antibody’s staining pattern in the PhenoCycler images.